Exploration of Active Metabolites from Aegle Marmelos and Emblica Officinalis for Determining its Immunosuppressive Properties

Abstract

As per the literature, bioactive compounds isolated or purified from medicinal plant products that are generally used for curing various human diseases. The present study involves two different medicinal plants i.e. Aegle marmelos and Emblica officinalis were locally available in Baramati region of Maharashtra, India. The leaves of these medicinal plants were used for phytochemical analysis in aqueous extract using phosphate buffered saline (PBS; pH 7.2) in order to find out the active metabolites using liquid chromatography mass spectrometry (LC-MS). For these studies, aqueous leaves extract of these two medicinally plants were tested for immunological studies in mice model studies especially Swiss mice pertaining to determine splenocyte proliferation assay using hepatitis B vaccine containing surface antigen (HBsAg) and also determined pro-inflammatory cytokines (IFN-gamma and TNF alpha) by Elisa. The results showed that aqueous leaves extract showed declined in splenocyte proliferation assay and proinflammatory cytokines (FN-gamma and TNF alpha) at higher doses as compared to control samples. HBsAg (20 µg/ml) used as standard for these studies. Overall, the results showed that aqueous leaves extract of Aegle marmelos and Emblica officinalis showed immunosuppressive activity against HBsAg as compared to control.