Laboratory evaluation of Aster 20SP against Culex pipiens (Diptera: Culicidae) immature stages
Abstract
The purpose of this research work was to investigate the larvicidal activities of Aster 20SP against Culex pipiens and biological effect of this compound. The larvicidal activities of Aster 20SP were tested against the third and fourth instar larvae of Culex pipiens, the mortality was observed after 24h post the treatment. The data were subjected to probit analysis to determine the lethal concentration (LC50 and LC90). The obtained results show a high mortality with the increase in the concentration, at third stage, larval mortality increased from 18.23 % at 0.7 mg /L to 45.16 % at 3mg /L in direct effect. The LC50 and LC90 were measured as 3.61 and 5.16 mg/L respectively. Cumulate mortality increased from 35.30 to 54.34% at 0.7mg/L and 3mg/L respectively. The LC50 and LC90 were 3.38 and 4.68mg/L respectively. At fourth stage, larval mortality increased from 5.50 % at 0.7 mg /L to 19.29 % at 3 mg /L in direct effect, the LC50 and LC90 was 4.34 and 6.52 mg/L respectively. In indirect effect, the mortality increased from 9.16% to 30.80% at 0.7mg/L and 3mg/L respectively, the LC50 and LC90 was 4 and 5.87 mg/L respectively. The results indicated the larvae duration increased with the increased in the concentration. In contrast, in adult stage, the results indicated high reduction in fecundity.
It was concluded from this study that Aster 20SP as a potential pesticide to control the larvae stage of mosquito Culex pipiens.Keywords
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Albernaz, D.A.S., Tai, M.H.H. and Luz, C. 2009. Enhanced ovicidal activity of an oil formulation of the fungus Metarhizium anisopliae on the mosquito Aedes aegypti. Med. Vet. Entomol, 23: 14–147.
Ali, A., Nayar, J.K. and Xue, R. 1995. Comparative toxicity of selected larvicides and insect growth regulators to Florida laboratory population of Aedes albopictus. J. Am. Mosq. Control Assoc, 11: 72–76.
Attardo, G.M., Hansen, I.A. and Raikhel, A.S.2005. Nutritional regulation of vitellogenesis in mosquitoes: Implications for anautogeny. Insect Biochem Mol Biol, 35(7): 661-675.
Berger, E.M. and Dubrovsky, E.B. 2005. Juvenile hormone molecular action and interaction during development of Drosophila melanogaster. Vitam. Horm, 73: 175–215.
Bernard, K.A., Maffei, J.G., Jones, S.A, Kauffman, E.B., Ebel, G.D., Dupuis,A.P, Ngo, K.A., Nicholas, D.C., Young, D.M., Shi, P.Y., Kulasekera, V.L., Eidson, M., White, D.J., Stone, W.B. and Kramer, L.D. 2001. NY State West Nile Virus Surveillance Team. West Nile virus infection in birds and mosquitoes, New York State, 2000. Emerg Infect Dis, 7: 679–685.
Cao, W., Van der Ploeg ,C.P., Ren Z. and Habbema, J.D.1997. Success against lymphatic filariasis. World Health Forum, 18: 17–20.
Cetin, H., Yanikoglu, A., Kocak, O. and Cilek , J.E .2006a. Evaluation of temephos and chlorpyrifos-methyl against Culex pipiens L. (Diptera: Culicidae) larvae in septic tanks in Antalya, Turkey. J Med Entomol 43: 1195–1199.
Cetin, H., Yanikoglu, A. and Cilek, J.E .2006b. Efficacy of diflubenzuron, a chitin synthesis inhibitor, against Culex pipiens larvae in septic tank water. J Am Mosquito Contr, 22: 343–345.
Chapagain, B.P., Saharan, V. and Wiesman, Z.2008. Larvicidal activity of saponins from Balanites aegyptiaca callus against Aedes aegypti mosquito. Bioresour Technol, 99(5): 1165-1168.
Dhar, R., Dawar, H., Garg, S., Basir, S.F. and Talwar, G.P .1996. Effect of volatiles from neem and other natural products on gonotrophic cycle and oviposition of Anopheles stephensi and An. Culicifacies (Diptera: Culicidae). J Med Entomol, 33:195–201.
Di Ilio, V., Cristofaro, M., Marchini, D., Nobili, P. and Dallai, R .1999. Effects of a neem compound on the fecundity and longevity of Ceratitis capitata (Diptera: Tephritidae). J Econ Entomol, 92:76–82
Govindarajan, M., Jebanesan, A. and Pushpanathan, T. 2008. Larvicidal and ovicidal activity of Cassia fistula Linn. Leaf extract against filarial and malaria vector mosquitoes. Parasitol. Res, 102: 289–292.
Gulia-Nuss, M., Robertson, A.E., Brown, M.R. and Strand, M.R.2011. Insulin like peptides and the target of rapamycin pathway co-ordinately regulate blood digestion and egg maturation in the mosquito Aedes aegypti. PLoS ONE; doi:101371/journalpone0020401.
Jacobson, M. 1987. Neem research and cultivation in Western hemisphere. In: Schmutterer, H., Ascher, KRS. (Eds.), Natural Pesticide from the Neem Tree and Other Tropical Plants. Proceedings of the 3rd Neem Conference, Nairobi, Kenya, pp: 33–44.
Jeyabalan, D., Arul, N. and Thangamathi, P. 2003. Studies on effects of Pelargonium citrosa leaf extracts on malarial vector, Anopheles stephensi Liston. Biores. Tech, 89 (2): 185 189.
Khan, M., Hossain, M.A. and Islam, M.S .2007. Effects of neem leaf dust and a commercial formulation of a neem compound on the longevity, fecundity and ovarian development of the melon fly, Bactocera cucurbitae (Coquillett) and the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). Pak J Biol Sci , 10:3656– 3661.
Lucantoni, L., Giusti ,F., Cristofaro, M., Pasqualini, L., Esposito, F., Lupetti, P. and Habluetzel, A .2006. Effects of a neem extract on blood feeding, oviposition and oocyte ultrastructure in Anopheles stephensi Liston (Diptera: Culicidae). Tissue Cell, 38:361–371.
Miura, T., Schaefer, C.H., Takahashi, R.M. and Mulligan, F.S. 1976. Effects of the insect growth regulator, Dimilin®, on hatching of mosquito eggs. J. Econ. Entomol, 69: 655–658.
Mordue (Luntz), A.J., Morgan, E.D. and Nisbet, A.J., 2005. Azadirachtin, a natural product in insect control. In: Gilbert, L.I., Iatron, K., Gill, S.S. (Eds.), Comprehensive Molecular Insect Science – Control, vol. 6. Elsevier, Oxford, United Kingdom, pp: 117–135.
Mulla, M.S. 1995. The future of insect growth regulators in vector control. J. Am. Mosq. Control Assoc, 11: 269–273.
Mulla, M.S. and Su, T. 1999. Activity and biological effects of neem products against arthropods of medical and veterinary importance. J. Am. Mosq. Control Assoc, 15: 133–152.
Musabyimana, T., Saxena, R.C, Kairu, E.W., Ogol, C.P.K.O. and Khan ,Z.R .2001. Effects on neem seed derivatives on behavioral and physiological responses of the Cosmopolites sordidus (Coleoptera: Curculionidae). J Econ Entomol, 94:449–454.
Muthukrishnan , J. and Pushpalatha, E.2001. Effects of plant extracts on fecundity and fertility of mosquitoes. J Appl Entomol , 125(1-2): 31-35.
Rajasekaran ,T. and Ravishankar, G. 2004. Obul Reddy B. In vitro growth of Tagetes patula L. hairy roots, production of thiophenes and its mosquito larvicidal activity. Indian J Biotechnol , 3: 92-96.
Sakthivadivel, M. and Thilagavathy, D.2003. Larvicidal and chemosterilant activity of the acetone fraction of petroleum ether extract from Argemone mexicana L. seed. Bioresour Technol , 89(2): 213-216.
Sarin , R. 2004. Insecticidal activity of callus culture of Tagetes erecta. Fitoterapia , 75(1): 62-64.
Saxena, R.C., Epino, P.B., Cheng-Wen, T. and Puma, B.C. 1984. Neem, chinaberry and custard apple: antifeedant and insecticidal effects of seed oils on leafhopper and planthopper pests of rice. In: Proceedings of 2nd International Neem Conference, Rauischholzhausen, Germany, pp: 403–412.
Saxena ,R.C., Harshan ,V., Saxena, A. and Sukumaran, P.1993. Larvacidal and chemosterilant activity of Annona squamosa alkabides against Anophel stephensi. J. Amer. Mosq .Control. Assoc , 9: 84-87.
Schmutterer, H. 1990. Properties and potential of natural pesticides from the neem tree, Azadirachta indica. Ann. Rev. Ent, 35: 271–297.
Soin, T., Swevers, L., Kotzia, G., Latrou, K., Janssen, C., Rouge, P., Harada, T., Nakagawa, Y. and Smagge, G. 2010. Comparison of the activity of non-steroidal ecdysone agonists between dipteran and lepidopteran insects, using cell-based EcR reporter assays. Pest Manag. Sci, 66: 1215–1229.
Staal, G.B.1975. Insect growth regulators with juvenile hormone activity. Annu Rev Entomol, 20: 417-460.
Steffens, R.J. and Schmutterer, H .1982. The effect of a crude methanolic neem (Azadirachta indica) seed kernel extract on metamorphosis and quality of adults of the Mediterranean fruit fly, Ceratitis capitata Wied. (Diptera: Tephritidae). Z Angew Entomol , 94:98– 103.
Su, T. and Mulla, M.S. 1998. Ovicidal activity of neem products (Azadirachtin) against Culex tarsalis and Culex quinquefasciatus (Diptera: Culicidae). J. Am. Mosq. Control Assoc , 14: 204–209.
Turell, M.J., O’Guinn, M .and Oliver, J. 2000. Potential for New York mosquitoes to transmit West Nile virus. Am J Trop Med Hyg, 62: 413–414.
Umar, A., Kela, S.L.and Ogidi, J.A. 2007. Ovicidal activity of neem (Azadirachta indica A. Juss) seed kernels extracted with organic solvents and distilled water on Aedes aegypti eggs. Int. J. Pure Appl. Sci. 1: 1–4.
Vasuki, V. 1990. Effects of insect growth regulators on hatching of eggs of three vector mosquito species. Proc. Indian Acad. Sci. Anim. Sci, 99 :477–482.
Weathersbee III, A.A. and Tang, Y.Q. 2002. Effect of neem extract on feeding, growth, survival, and reproduction of Diaprepes abbreviatus (Coleoptera: Curculionidae). J Econ Entomol , 95:661–667.
WHO. 2005. Report of the eight WHOPES working group meeting. Review of: Novaluron 10% EC.WHO/CDS/WHOPES/.10.
WHO [World Health Organization]. 2006. Pesticide and Their Application. For the Control of Vectors and Pests of Public Health Importance. Geneva, Switzerland. WHO/CDS/NTD/WHOPES/GCDPP/.1.
Zahiri, S.N. and Mulla, M.S. 2006. Ovipositional and ovicidal effects of the microbial agent Bacillus thuringiensis israelensis on Culex quinquefasciatus Say (Diptera: Culicidae). J. Vector Ecol, 31: 29–34.
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