Efficient DNA Extraction Technique from Leave Tissues of Some Important Tropical Plant Species

Kapilan Ranganathan

Abstract


ABSTRACT

Extraction of DNA is very important aspect in the plant molecular biological investigations. In this study, a large number of samples such as fresh leaves obtained from different plant species Oryza sativa, Elusine korakkana, Zea mays, Azadirachta indica, Musa sapianatum, Sesbania grandiflora were taken and faster method of Lin Rong et al (2001) applied for DNA extraction that can provide huge amount of high quality DNA determined by horizontal agarose gel electrophoresis using 1% agarose in TBE buffer at constant voltage of 60V. Purity was measured by the ratio of 260/280nm near to 1.8 by using Nanodrop ND-1000 spectrophotometer.


Keywords


DNA extraction, quality, purity

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References


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